The role of gender in the selection of human resources has been investigated in the area of human resource management. Since this discussion was raised using a non-religious approach, the selection of women in all jobs is done without any special condition. Today, in Islamic societies, women have various jobs, while their presence at the workplace causes them to deal with the Non-Mahrams. Therefore, it is necessary to examine women's jobs from the perspective of Islam. Using the "descriptive and historical" method, the present research tries to investigate the role of gender in the selection of human resources with regard to the life of the Holy Prophet and Imam Ali. What is obtained from examining the life of the Prophet and Imam Ali is that women were active in various scenes and no prohibition was issued by them. Therefore, there is no prohibition to select women in various jobs as long as Islamic rules are observed in the work environment and organization. If Islamic laws are violated, it is forbidden for women to work.